The Role of Intracellular pH in Fertilization of Sand Dollar Eggs Analyzed by Microinjection Method. (intracellular pH/fertilization/microinjection/pH indicator/pH buffer)
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چکیده
منابع مشابه
Simultaneous investigation of intracellular Ca2+ increase and morphological events upon fertilization in the sand dollar egg.
An increase in intracellular Ca2+ concentration ([Ca2+]) and morphological were simultaneously observed by epifluorescence and differential interference contrast (DIC) microscopy during fertilization of the sand dollar, Clypeaster japonicus. [Ca2+], which was detected by a Ca2+ indicator, Fluo-3, initially increased just beneath the sperm-attached site on the egg surface 8.6 sec after attachmen...
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Intracellular calcium in the fertilization and development of mammalian eggs.
1. Mammalian eggs are arrested at metaphase of their second meiotic division when ovulated and remain arrested until fertilized. The sperm delivers into the egg phospholipase C (PLC) zeta, which triggers a series of Ca(2+) spikes lasting several hours. The Ca(2+) spikes provide the necessary and sufficient trigger for all the events of fertilization, including exit from metaphase II arrest and ...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1982
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1982.00443.x